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KMID : 0545120000100050628
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 5 p.628 ~ p.631
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Increased Poly(3-Hydroxybutyrate)Accumulation in Recombinant Escherichia coli from Whey by Agitation Speed Control
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KIM, BEOM-SOO
O´NEILL, BRIAN K./LEE, SNAG-YUP
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Abstract
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The timing of poly(3-hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15g/l. When the agitation speed was increased up to 500rpm, a cell concentration of 31g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.
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KEYWORD
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